| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1235-2 | ||||
Resumo:Candidiasis is a prevalent infection caused by the excessive growth of textoCandidatexto species, particularly textoCandida albicanstexto, an opportunistic yeast that is part of the human resident microbiota. However, factors such as the irrational use of antimicrobials and issues with the immune system can trigger the uncontrolled proliferation of this fungus. While candidiasis can be treated with antifungal agents, the escalating resistance to these medications presents significant therapeutic challenges. Hence, the quest for new therapies is imperative. In this context, vegetable bio-oil emerges as an appealing, sustainable option with a diverse chemical composition that may enhance its potential as a therapeutic agent. The aim of this study was to assess the antifungal potential of bio-oil (BO) derived from the pyrolysis of residual wood from Citrus sinensis against textoCandida albicanstexto. The residual wood from C. textosinensistexto was collected from a commercial plantation located in the municipality of Jerônimo Monteiro, Espírito Santo, Brazil. Subsequently, the material was ground and subjected to the pyrolysis process in a muffle furnace, coupled with a gas condenser, using a final temperature of 450°C, a heating rate of 7°C/min, and a residence time of 30 minutes. The collected bio-oil (BO) underwent a double distillation process to remove tar, a potentially toxic product, resulting in purified bio-oil (BOP). The identification of chemical compounds present in the BOP was performed using gas chromatography-mass spectrometry (GC/MS - Agilent Technologies 7820A GC/5977B MSD system). The evaluation of the antifungal activity of BOP was conducted using the standard strain of textoCandida albicanstexto (ATCC 24433) through minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) tests. These tests were conducted following the guidelines of the Clinical and Laboratory Standards Institute (CLSI) M27-A3 from 2008. The BOP exhibited a diverse chemical composition, comprising 49 distinct chemical components distributed across various classes, including carboxylic acids, furans, pyrans, alcohols, ketones, and phenolic compounds. Acetic acid, furfural, and 2-methoxyphenol (o-guaiacol) stood out as the most abundantly present substances in terms of percentage area in the BOP. A concentration of 1.25% of BOP was determined as the effective MIC and MFC against the standard C. textoalbicanstexto strain, demonstrating highly effective antifungal activity. This remarkable antifungal activity can be attributed to the synergistic action of the various chemical compounds present in the BOP, particularly the high concentration of phenolic compounds identified in the sample. These results highlight the therapeutic potential of C. textosinensistexto BOP in the treatment of candidiasis, encouraging future research. Furthermore, the investigation of natural products like BOP strengthens the pursuit of sustainable therapies, not only for candidiasis but also for other medical conditions, promoting a healthier and more ecological future. Palavras-chave: antifungal activity, Candida albicans, pyrolysis, waste wood Agência de fomento:Fundação de Amparo à Pesquisa e Inovação do Espírito Santo | |||||